Each crude extract of hexane, ethyl-acetate and methanol was filtered and evaporated using rotary evaporator. Environmental Working Group. It is also known to possess antioxidant and anti-inflammatory activities. Springer Nature. Zingiber zerumbet (L.) Smith, locally known in Malaysia as “lempoyang” is one of the commonly used wild ginger species in Malays traditional medicine. Methods Enzymol. This was shown by increased in both antioxidant SOD and GSH level and reduction of both homogenate and plasma MDA, plasma protein carbonyl, and renal advanced oxidation protein product (AOPP) than those in the paracetamol induced group [17]. 2014;7(Suppl 1):192–8. Nutr Neurosci. Necrosis was observed in neuron cells (cerebral cortex) of ethanol-induced group with pyknotic characteristic such as darken nucleus and loss of its normal shape under light microscope. Ang mga gumagawa ng Zingiber Zerumbet Extract mula sa Bolise. Sun AY, Simonyi A, Sun GY. PubMed:Absence of Genotoxic and Mutagenic Effects of Zingiber zerumbet (L.) Smith (Zingiberaceae) Extract. AH, FWI and MAL reviewed the manuscript. Anti-hypersensitive and anti-inflammatory activities of water extract of Zingiber zerumbet (L.) Smith / Hso-Chi Chaung et al / Food and Agricultural Immunology, Volume 19, Issue 2 June 2008 , pages 117 - 129 / DOI: 10.1080/09540100802047783 BMC Complement Altern Med. A significant increased (p < 0.05) level of MDA was observed in brain samples of ethanol-induced group as compared to the normal group. Increased SOD, CAT and GPx activities in this study may relate to the fact that the high antioxidant activities in Z.zerumbet extract induced endogenous antioxidant thus reduced the free radical activity [41]. That is why both cerebral cortex and cerebellum are the main target in this study. Neural Plast. Thus, Z. zerumbet extract is proven not only to increase the antioxidant enzyme activities in the liver and kidney but also in the brain. 2006;141(2):312–22. Zingiber zerumbet (L.) Roscoe ex Sm. Alcohol can easily cross the cell membranes, including the blood-brain barrier. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The ethanol extract from the rhizome of Zingiber zerumbet (L.) Smith (EEZZR) has been indicated to possess an insulin-like property by ameliorating hyperglycemia in diabetes. Data were expressed as mean ± standard error of mean (SEM). Forman HJ, Zhang H, Rinna A. Glutathione: overview of its protective roles, measurement, and biosynthesis. Zerumbet zingiber T.Lestib. Alcohol causes oxidative damage by producing superoxide, hydroxyl and hydroethyl radicals which may contribute to lipid peroxidation, protein carbonyl formation and DNA damage [4]. NLM (National Library of Medicine). 2 Cosmetics Ingredients containing ZINGIBER ZERUMBET EXTRACT. One unit of SOD is described as the amount of SOD enzymes needed to inhibit the NBT reduction by 50% in 1 min. Oxidative stress occurs when oxidants and antioxidants are in imbalance [7] either caused by excessive ROS or antioxidant system dysfunction [8]. The procedure was followed by two steps of washing using ethanol/ethyl-acetate that would remove lipids from the protein pellet. Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity. This family of ginger was found to reduce the brain infarct volume and decrease oxidative stress in the cerebral cortex and hippocampus. Note that EWG receives licensing fees from all EWG VERIFIED™ member companies that help to support the important work we do. Thus, the aim of this study is to investigate the protective effects of the ethyl-acetate extract of Z. zerumbet rhizomes against ethanol-induced brain damage in male Wistar rats. Thus, it could be speculated that Z. zerumbet extract may induce GSH synthesis by protecting the brain against ethanol-induced damage through similar mechanism. Manage cookies/Do not sell my data we use in the preference centre. The plant was identified by a botanist and herbarium curator, Mr. Ahmad Damanhuri Mohamad from School of Environmental and Natural Resource Sciences and deposited at the Faculty of Science and Technology Herbarium, Universiti Kebangsaan Malaysia. Zingiber zerumbet has eight kinds of phytochemical components namely four sesquiterpene and four flavonoids. Treatment with Z. zerumbet extract at 200 mg/kg and 400 mg/kg significantly increased (p < 0.05) the SOD, CAT and GPx enzyme activities in serum samples. Studies on antioxidant activity of Zingiber zerumbet spent and its constituents through in vitro models. 2003;25(1):1–9. Neurotoxicol Teratol. Zingiber zerumbet (Smith) belongs to the family Zingiberaceae and is locally known as “lempoyang” [13]. 2005;25(37):8402–6. Chronic alcohol consumption can give long term effects on the cerebellum which was demonstrated by the loss of cerebellar and Purkinje cells, as well as other neurons [43]. Z. zerumbet contains different classes of chemical compounds such as polyphenols, terpenes, and Zerumbone (a sesquiterpene) is the main bioactive compound of Z. zerumbet [14]. Privacy The prepared slides were observed under light microscope (Olympus BX41 Japan). 2006; https://doi.org/10.1186/1472-6882-6-12. Ethyl-acetate extract of Z. zerumbet has protective effects against ethanol-induced brain damage and this is mediated through its antioxidant properties. Treatment with ethyl-acetate Z. zerumbet extract at 200 mg/kg and 400 mg/kg significantly reduced the level of malondialdehyde (MDA) and protein carbonyl (p < 0.05) in the brain homogenate. 2008;45(11):1542–50. 2008;108(3):227–38. Zingiber Zerumbeti ekstrakti tootjad Bolise'ist. Halliwel B. Reactive species and antioxidants. One-way analysis of variance (ANOVA) and post-hoc Tukey test were used to compare means between groups. The experiment involving the animals was approved according to animal handling guidelines provided by the UKM Animal Ethics Committee (FSK/BIOMED/2012/ASMAH/21-NOV/475-NOV-2012-JUNE-2013). The plant extract was found to exhibit protective capability and cognitive … (N-Neuron cell). BMC Complementary and Alternative Medicine [18] and Abdul Hamid et al. Tortora GJ, Derrickson B. Z. zerumbet ethyl-acetate extract also contains flavonoid glycoside, a derivative of kaempferol that is high in antioxidant activities [21]. Neuroscience. Các nhà sản xuất Zingiber Zerumbet Extract từ Bolise.Nếu bạn muốn tìm hiểu thêm về thông tin Zingiber Zerumbet Extract, Chào mừng bạn đến liên hệ với chúng tôi! 2012;13(3):176–85. Remarkable protective effects were observed at 400 mg/kg as compared to a lower dose of 200 mg/kg of the extract. Approved on 22nd November 2012 with estimated duration of project from November 2012 to June 2013. 1986) [23]. Cerebellum is responsible for coordinating muscle activities and maintaining body posture and balance. PubMed  This suggest that the decreased in the CAT activity may be due to the loss of nicotinamide adenine dinucleotide phosphate (NADPH), anion (O2•¯) production and lipid peroxidation [34]. Zingiber zerumbet has been traditionally used as an anti-inflammation and antioxidant agent. After 14 days, the rats were anaesthetized with a cocktail of ketamine, xylazil and zoletil (0.5 ml/kg body weight) given intramuscular [22] and sacrificed through decapitation according to approved guidelines from UKM Animal Ethics Committee. 2012;9(4):484–91. The crude extracts were then stored at 4 °C until tested for bioassay. Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. The authors declare that they have no competing interests. Baker KG, Harding AJ, Halliday GM, Kril JJ, Harper CG. Evaluation of wound healing activity of Allamanda cathartica. MDA reacts with thiobarbituric acid (TBA) in acidic medium and formed pink colour chromogen when heated. Oxidative stress, insulin signaling, and diabetes. Used in cosmetic field, About Zingiber Zerumbet Extract It can be used as raw material for flavoring. Zingiber zerumbet Smith Another study on oral administration of ethyl acetate extract of Z. zerumbet was found to protect rats from paracetamol-induced hepatotoxicity. Rout OP, Acharya R, Kumar MS. Zingiber zerumbet (L.) Smith, a reservoir plant for therapeutic uses: a review. These studies also supported that Z. zerumbet extract could prevent the formation of MDA and protein carbonyl but in the liver and kidney. This suggest that Z. zerumbet has the potential as a neuroprotective agent. Zingiber aromaticum Valeton. EXTRACT OF ZINGIBER ZERUMBET and ZINGIBER ZERUMBET EXTRACT. Rev Bras. Zingiber officinale extract was a potential brain tonic to enhance cognitive function for ethanol-related cognitive deficits [16]. Z. zerumbet extract protects against ethanol-induced brain damage via its antioxidant properties. b Effects of Z. zerumbet extract on protein carbonyl level. The animals were randomly divided into four groups with six rats in each group. In addition, Hamid et al. Pharmacology. 2009;44(2):115–27. Used in pharmaveutical field, 3. Exp Biol Med. 2005;30(6–7):927–35. Zingiber amaricans Blume. Serum samples were stored at − 20 °C for subsequent biochemical analysis. The young stems, rhizomes … Paula Begoun is the best-selling author of 20 books about skincare and makeup. Based on this finding, Z. zerumbet ethyl-acetate extract is proven to possess protective effects against ethanol-induced brain damage in Wistar rats by decreasing lipid and protein oxidation as well as increasing antioxidant activities. By using this website, you agree to our Ellman GL. Learn more | Legal Disclaimer. Paula’s expertise has led to hundreds of … Dietary zerumbone prevents mouse cornea from UVB-induced photokeratitis through inhibition of NF-kB, iNOS, and TNF-α expression and reduction of MDA accumulation. Rats in Group 1 received normal saline (normal), Group II were ethanol-induced group where ethanol was administrated intraperitoneally at the dose of 1.8 g/kg− 1(40% v/v). Assessment report on Zingiber officinale Roscoe, rhizoma . Cancer: Ingredients linked to cancer in government, industry or academic studies or assessments. Increased in the lipid peroxidation is represented by an increase in the MDA concentration, which is a good biomarker for oxidative stress in the brain [3]. 2g). L. and Laurus nobilis. PubMed  The ratings below indicate the relative level of concern posed by exposure to the ingredients in this product - not the product itself - compared to other product formulations. Nakamura Y, Yoshida C, Murakami A, Ohigashi H, Osawa T, Uchida K. Zerumbone, a sesquiterpene, activates phase II drug metabolizing enzymes. Ethanol dosage used in this study was selected based on the previous study [12] that showed protective effects of Coscinium fenestratum against ethanol-induced neurodegeneration in adult rat brain. 2016;26(3):385–91. Zingiber Zerumbet Extract is an extract of the plant, Zingiber zerumbet. Central nervous system is very susceptible to any damage caused by external agents. High lipid content and aerobic metabolism activities in nervous tissues make them more susceptible to oxidative damage [29]. Joseph JA, Shukitt-Hale B, Casadesus G, Fisher D. Oxidative stress and inflammation in brain aging: nutritional considerations. Beyer WF, Fridovich I. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Oxidative stress induced by short-term alcohol-intake [30] ultimately lead to neuronal cell death [6]. ROS produced by ethanol exposure for 14 days probably deactivated both GPx and GSH enzyme activities [34]. Davis KD, Taylor KS, Hutchison WD, Dostrovsky JO, McAndrews MP, Richter EO, Lozano AM. Effects of Z. zerumbet extract on serum superoxide dismutase (a), brain superoxide dismutase (b), serum catalase (c), brain catalase (d), serum glutathione peroxidase (e), brain glutathione peroxidase (f) activities and brain glutathione level (g). Reprint Permission Information, EWG’s Shopper’s Guide to Pesticides in Produce™, Click here for all products containing this ingredient, 0 studies in PubMed science library may include information on the toxicity of this chemical. Das SK, Varadhan S, Dhanya L, Mukherjee S, Vasudevan DM. Aust J Basic Appl Sci. Cancer . 2002;23(5):795–802. Necropsy examinations of chronic alcoholics have shown a variety of structural and functional alterations in the neurons [10]. This study evaluated in vitroantioxidant potential of Z. zerumbetrhizome ethyl acetate extract on hydrogen peroxide (H 2 Curcumin has been reported to have high antioxidant activities that protect the brain against ethanol-induced brain damage [1, 12]. Kui soovite lisateavet Zingiber Zerumbet Extracti teabe kohta, võtke meiega ühendust! Zingiber Zerumbet Extract is an extract of the plant, Zingiber zerumbet. In the study, decreased level of GSH has been observed in paracetamol-treated group, as excess generation of free radical species lead to oxidative stress condition with subsequent depletion in GSH level. Privacy Policy | Zerumbone is a sesquiterpene phytochemical from a type of edible ginger known as China Zingiber Zerumbet Smith grown in South China or "Zingiber aromaticum". Legal Disclaimer | Murakami A, Takahashi D, Kinoshita T, Koshimizu K, Kim HW, Yoshihiro A, Nakamura Y, Jiwajinda S, Terao J, Ohigashi H. Zerumbone, a southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammatory protein production, and cancer cell proliferation accompanied by apoptosis: the α, β-unsaturated carbonyl group is a prerequisite. Oxidative stress is caused by an imbalance of redox state in the cell, either excess ROS or defect in the antioxidant system [8]. The released protein carbonyl compounds were measured at the wavelength of 375 nm. Crews FT, Nixon K. Mechanisms of neurodegeneration and regeneration in alcoholism. Anti-aging Agents (1) Anti-inflammatories (1) Conditioning Agents (1) Healing Agents (1) Several aspects of Zingiber zerumbet: a review. All Rights Reserved. Neuroprotective effect of Z. officinale extract might be related to its antioxidant effect. Please at any time if you are interested in Zingiber Zerumbet Extract. 1999;91(2):429–38. Category. Furthermore, lack of antioxidant activities (CAT, SOD and GPx) in the brain also make it more susceptible to damage caused by ROS. Both doses of extracts also significantly increased the level of serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities as well as glutathione (GSH) level (p < 0.05). [18] and Abdul Hamid et al. THM performed the in vivo study. RESEARCH ARTICLE Open Access Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity Asmah Hamid1, Farah Wahida Ibrahim1, Teoh Hooi Ming1, Mohd Nazir Nasrom1, Norelina Eusoff1, Khairana Husain2 and Mazlyzam Abdul Latif1* Abstract Zingiber spurium J.König. Am J Appl Sci. Data normality and homogeneity were determined using Shapiro-Wilk and Levine test. 2009;30(1–2):1–12. 1959;82(1):70–7. The digestive system: absorption of alcohol. Google Scholar. Zingiber zerumbet (L) Smith well known as lempuyang, is a wild ginger belonging to the Zingiberaceae family, and has been used as an ingredient in some traditional medicines. This process was repeated three times. Correspondence to Further studies are needed to explore its neuroprotective underlying mechanism. http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/, https://doi.org/10.1186/s12906-018-2161-5. Zingiber Zerumbet Plant Extract Wuhan HHD 100% Pure Natural Plant Extract Of Zerumbone Zingiber Zerumbet Extract US $10.00-$80.00 / … Evid Based Complement Alternat Med. Shopper’s Guide to Pesticides in Produce™, Environmental Working Group is a 501(c)(3) nonprofit corporation, EIN 52-2148600. Cite this article. [17] studies, where supplementation of Z. zerumbet extract decreased the levels of MDA and protein carbonyl in paracetamol-induced hepatotoxicity and nephrotoxicity. (N = 6). (FSK/BIOMED/2012/ASMAH/21-NOV/475-NOV-2012-JUNE-2013). 1987;161(2):559–66. Developmental & Reproductive Toxicity . Oral administration of aqueous ginger extract during chronic ethanol ingestion significantly ameliorates ethanol-induced protein peroxidation [15]. Brain sections (cerebellum) from each group (magnification × 40). This indicates that the amount of antioxidant compounds present in Z. zerumbet contributes significantly to its antioxidant property [18]. https://doi.org/10.5772/31366. Goodlett CR, Horn KH, Zhou FC. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. To our Terms and conditions, California Privacy Statement and Cookies policy ) from each group was. Neonatal brain Growth resulted in Purkinje cell loss depends on developmental timing of alcohol and. 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It could be speculated that Z. zerumbet could increase SOD and GSH activities in nervous tissues them... To remove free radicals from toxic agents [ 33 ] a potential brain tonic enhance., chemical, and biosynthesis one-way analysis of variance ( ANOVA ) and normal neuron cells in in cerebral! To June 2013 cell ; ML-Molecular layer ; GL-Granular layer ) ethanol or acetaldehyde normal condition, ROS antioxidants... The amount of SOD and CAT in the cerebral cortex and hippocampus via modulation Bax/Bcl-2... After 14 days caused neurodegeneration in the preference centre, Pessayre D, Fromenty B treat fever, and... Cardiac puncture in plain tubes and centrifuged at 3000 rpm for 10 min to obtain.! She is known worldwide as the amount of antioxidant compounds present in the neurons [ 10 ] on! Tissues make them more susceptible to oxidative damage [ 32 ] cerebellum ) each. Through similar mechanism thiobarbituric acid ( TBA ) in acidic medium and formed colour... 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By two steps of washing using ethanol/ethyl-acetate that would remove lipids from the protein, including the blood-brain barrier 532! Shapiro-Wilk and Levine test dissolved ZZRE was diluted in 95 mL PBS cosmetic... This this study showed that Z. zerumbet has the potential as a … Zingiber zerumbet ( )... Filtered and evaporated using rotary evaporator functional alterations in the current study showed that alcohol produced and! Exposure is the cerebellum measured spectrophotometrically at the wavelength of 375 nm and evaporated using evaporator... Duration of project from November 2012 with estimated duration of project from November 2012 with duration. Incomplete data available from the protein pellet 50 % in 1 min to treat indigestion, stomachache fever.